MyTaq™ HS DNA Polymerase


* New generation of antibody-based hot-start polymerase
* Highest specificity and superior performance
* Novel buffer system, including dNTPs and MgCl2
* Fast PCR reactions


* High-throughput PCR
* Assays with prolonged reaction setup on the bench or liquid handling
* Amplification of challenging targets susceptible to mispriming
* Colony PCR
* Multiplexing
* Specific amplification of difficult templates (GC rich)
* Genotyping
* TA cloning
* Two-Step RT-PCR


MyTaq™ HS DNA Polymerase is a high performance PCR product powered by antibody mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. The product also has the added convenience of room temperature reaction assembly, without non-specific amplification and primer-dimer formation. This new hot-start enzyme preparation from Bioline is supplied with 5x MyTaq Reaction Buffer, a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification.

Unit Definition:

One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.



Storage Conditions

MyTaq HS DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.


Cat No. Size Conc.
BIO-21111 5u/µl 250 Units
BIO-21112 5u/µl 1000 Units
BIO-21113 5u/µl 2500 Units

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