VELOCITY DNA Polymerase:


# High-speed, high fidelity DNA polymerase
# High processivity
# Fast amplification
# Shorter PCR runs for longer templates
# Robust, requires minimal optimization of the reaction


# Cloning techniques where high fidelity is desirable
# Blunt-end cloning
# Amplification of difficult templates
# Mutagenesis


VELOCITY is a fast thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities. VELOCITY provides high fidelity with an error-rate of 4.4 x 10-7 combined with a high speed DNA synthesis as fast as 15s/Kb for templates of up to 5Kb and 30s/Kb for templates longer than 5Kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase

VELOCITY is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of VELOCITY DNA Polymerase (4.4 x 10-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).

Working Concentration:

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.



Storage Conditions

ELOCITY DNA Polymerase can be stored for 12 months at –20°C

Shipping Conditions

On Dry Ice or Blue Ice


Cat No. Product Units Price
BIO-21104 VELOCITY PCR Kit 250 Units, 20 reactions 230 EUR
BIO-2109 Velocity DNA Polymerase 250 Units 195 EUR
BIO-21099 Velocity DNA Polymerase 500 Units 360 EUR

"I have used VELOCITY for amplifying Illumina small RNA libraries and it works better than the Illumina-supplied Phusion enzyme with less primer-dimer formation and also for amplifying a 4.7Kb promoter from tomato gDNA which worked much better than either KOD Hotstart or PfuUltra Fusion HS polymerase enzymes."

K. Manning, Warwick HRI, Wellesbourne, Warwick, UK.

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